Introduction: The aim of the research was the effect of citrulline malate supplementation and TRX exercises on muscle strength, insulin-like hormone and myostatin in male bodybuilders in Tehran. + supplement, 3: supplement, 4: control) were divided. The criteria for entering the research included: being male, age 20 to 30 years, volunteering to participate in the research, not smoking and not consuming alcoholic beverages. The criteria for exiting the research were also It included unwillingness to participate in the research, having an illness at the time of conducting the research, having an injury during the implementation of the exercise, performing heavy physical activity outside the research within 72 hours before participating in the test. and physical activity and sampling) along with its objectives and possible risks were explained to the subjects and their demographic information was obtained from them along with written voluntary consent. The day after this meeting, the subjects were asked to come to the Isak Badi Niavaran sports club to measure a maximum repetition. 48 hours after the test of a maximum repetition, the first blood sampling was taken at 8:00 a.m. and in the state of 12 hours of overnight fasting from all the subjects. It was taken in the post-test. The supplement group took 2 grams of citrulline malate in the form of capsules from Seven Nutrition Company with lunch. The TRX training group performed the exercises for eight weeks. The analysis and analysis of the data was done by the analysis of variance with 2x2 repeated measures at a significance level of 0.05. Findings and results: The results showed that the training and supplement and training alone It had a significant effect on the levels of IGF-1, myostatin and muscle strength (p<0.05). Conclusion: citrulline malate supplement loading and TRX training have a significant effect on the anabolic status of male bodybuilders.