Aging is a process that causes a decrease in biological functions and increases the probability of cardiovascular disease. Also, the heart muscle, as an oxidative tissue with continuous activity, is one of the tissues prone to oxidative damage that occurs under oxidative stress conditions. Free radicals cause the production of substances such as malondialdehyde (MDA), which catalase (CAT) plays an important role in modulating the conditions of oxidative stress. The purpose of the research was to determine the effect of six weeks of high-intensity interval training and 10-Q supplementation on the amount of catalase and malondialdehyde in the heart tissue of aged male Wistar rats. In this research, 17 elderly male laboratory rats with an average age 22 months and an average weight of 422 grams were randomly divided into five groups (control, sham exercise, sham supplement, high-intensity interval training and high-intensity interval training+supplement group). The high-intensity interval training protocol 5 days a week A period of six weeks was designed for the training groups. The training group was given coenzyme Q10 supplement dissolved in sesame oil 20 mg/kg daily according to the average weight of the research mice for six weeks by gavage. 48 hours after In the last practice session, the samples were anesthetized with a combination of xylazine and ketamine, and heart tissue was extracted. The ELISA method was used to measure MDA and CAT. Descriptive statistics were used to analyze and analyze the data, and to check the normality of the data distribution, the method was used. Shapiro-Wilk was used. Due to the normality of the data, one-way analysis of variance and Sheffe's post hoc test were used at the significance level (P≤0.05). Calculations were done using SPSS software, version 26. The findings of this research showed that six weeks of high-intensity interval training along with 10Q supplementation led to a significant increase in CAT enzyme activity and a significant decrease in MDA in the heart tissue of aged male Wistar rats (P≤0.05). According to the findings of this research, it can be concluded that high-intensity interval training along with 10Q supplementation, with a decrease in MDA and a significant increase in CAT enzyme activity, probably prevents the weakening of the heart's antioxidant system following aging and oxidative damage in Reduces heart tissue. According to the results, it is suggested to use high-intensity interval training along with Q10 supplement to prevent oxidative stress and MDA accumulation and increase CAT antioxidant capacity in heart tissue.
Keywords: high-intensity interval training, oxidative stress, 10Q, antioxidant agents, aging