Type 2 diabetes is one of the most common metabolic diseases in recent years, which has increased with the exponential increase in obesity and has become a risk factor in the occurrence of cardiovascular diseases and one of the main causes of death worldwide. Therefore, the aim of this study is to investigate the effects of high-intensity interval training on changes in the levels of mTOR protein associated with physiological and pathological hypertrophy in the heart tissue of male Wistar rats with type 2 diabetes. In this experimental research, 40 eight-week-old male Wistar rats with an average weight of 191/3±16/9 grams were randomly divided into four healthy control groups, control diabetes exercise and exercise diabetes group, each group includes 10 rats. Diabetes was induced in animals of diabetic groups by high-fat diet and intraperitoneal injection of STZ. The exercise program included 8 weeks of high-intensity interval training with a sequence of 5 sessions per week. The training intensity was calculated based on the percentage of the maximum speed estimation test that was taken before the main training, which was set in the range of 80% of the maximum speed in the first week to 100% of the maximum speed in the final week, 48 hours after the last training session and after Anaesthetized under sterile conditions, heart tissue was isolated and the levels of desired protein were measured using western blot method. One-way statistical method and Tukey's post hoc test were used to check the difference between groups in each variable. SPSS version 25 and Excel 2016 software were used for data analysis. A significance level of P<0.05was considered. The results of this study showed that the induction of diabetes caused a significant decrease in the amount of mTOR protein in the heart tissue of diabetic rats (P<0.05). Also, eight weeks of high-intensity interval training caused a significant increase in the amount of mTOR protein in the heart tissue of diabetic rats (P<0.05). According to the obtained results, it can generally be said that intense interval training has a beneficial role in regulating and Control of signaling pathways related to physiological hypertrophy and inhibition of pathological hypertrophy in samples with type 2 diabetes It has two and could possibly be promising as a non-pharmacological strategy.