Non-alcoholic fatty liver disease is caused by excessive accumulation of triglycerides in the liver. NAFLD leads to inflammation and oxidative stress in the body, it seems that due to oxidative stress, the expression of sirtuin 6 is disturbed and telomeres are eroded. Therefore, the purpose of this research is to investigate 8 weeks of High interval training (HIIT) and Portulaca oleracea L on SIRT6 levels. Materials and methods: For this purpose, 20 adult male Wistar rats, six weeks old and weighing between 160 and 185 grams. They were randomly selected and placed in 4 groups: healthy control, fatty liver control, purslane supplement consumption, HIIT exercise and exercise with purslane supplement consumption. To induce NAFLD, rats were fed a high-fat diet for 12 weeks. Purslane supplement was given to the respective groups according to the weight of the mice at a dose of 400 mg/kg. The HIIT training protocol was performed for 8 weeks, 5 days a week, with 7 repetitions of one minute with an intensity of 90% of the maximum speed, which was accompanied by periods of active rest including 2 minutes of running with an intensity of 20% of the maximum. The amount of SIRT6 was measured by western blotting. One-way analysis of variance were used to analyze the data.
Findings: 8 weeks of High-intensity interval training with Portulaca oleracea L supplementation did not show any significant difference in the level of sirt6 , P=0.664.
Conclusion: 8 weeks of high intensity interval training with Portulaca oleracea L extract did not increase sirtuin6 levels