Introduction: The increasing prevalence of obesity has affected millions of people in the world, which will lead to various diseases such as pre-diabetes, diabetes, cardiovascular diseases, cancer and muscle disorders. Pre-diabetes has become a problem in many developed and non-developed countries, and actions in pre-diabetes people will be effective in reducing the number of diabetics. The aim of the present study was to investigate the effect of aerobic exercise on the gene involved in skeletal muscle volume (LRRC8A) in the twin muscle of pre-diabetic rats.
Materials and methods: 15 male C57BL/6 mice were randomly divided into two groups: mice fed with a normal diet (ND, 5) and high-fat diet (HFD, 10). Mice were fed for 12 weeks. After detecting the induction of prediabetes by diagnostic tests in the HFD group, this group was divided into two subgroups: prediabetic mice (PD, 5 heads) and prediabetic mice with aerobic exercise (PD-Ex, 5 heads), which It was done by running on a treadmill 5 days a week for 45 minutes every day and with an increasing speed from 15 to 23 meters per minute.
Research findings: A significant increase in blood glucose level and insulin resistance was observed in the HFD group compared to ND, as well as a significant decrease (P<0.0001) in blood glucose level and insulin resistance in the PD-Ex group compared to PD. The expression level of LRRC8A gene showed a significant decrease (P<0.05) between the PD group compared to the ND group. This indicates a disturbance in biological processes such as proliferation, migration, growth and cell death in muscles. On the other hand, aerobic exercises led to a significant improvement in the level of LRRC8A gene expression.
Discussion and conclusion: It seems that performing aerobic exercises improves the negative effects of pre-diabetic conditions in the muscle tissue of pre-diabetic rats.