Nowadays soccer has been as a popular field among the societies. furthermore, soccer attracts millions of television spectators while the economic impact of elite soccer, affects almost each culture around the world and therefor, soccer studies can significantly affect improvement in each component. The research articles were searched for and selected from electronic databases, including Medline, Scopus, PubMed, web of science, and ScienceDirect. There were no filters applied to the soccer players’ level, race, or age to increase the power of the analysis. The length of the studies ranged from 6 weeks to 12 weeks. The literature search identified a total of (N=189) papers related to the topic, but only 4 articles had all the inclusion criteria. The participants included in the systematic review (N=124), who competed in professional or elite (N=64), and young players (N=60). The supplement dosage also varied from study to study, ranging from 5000 to 6000 IU per day. and also, a paper with 200\000 IU Single dosage. The mean concentration of four studies at baseline was 31.475 ng/mL. We found that there is strong relation between VD deficiency and reduction of male soccer players. There are significant negative correlations between the 25(OH)D concentration sprint times on the distances of 5 m, 10 m, and 30 m. there isn’t any relation between the level of VD serum concentration and aerobic capacity. there isn’t any relation between the level of VD serum concentration and aerobic capacity. The effect of VD supplementation on physical performance of male soccer players should be examined in different soccer positions (like Right Midfielder or Winger) and also, post researches should investigate the effect of VD status on muscle strength of male soccer players.