The ability to acutely enhance sports performance is a competitive advantage for athletes. With the phonemenon of post activation potentiation, athletes can achieve this ability. This study aimed to evaluate the post activation potentiation (PAP) effect of hip thrust exercise on sprint performance in sprinters. Ten male sprinters (mean ± SD, age 24.90±2.60 years, weight 75.80±18, height 182.20±8.25, BMI 22.8 ± 1.22, and training experience 4.10±0.994 years) were involved in a randomised, crossover study. To evaluate the post activation potentiation effect of hip thrust exercise, a 30-metre sprint test was performed. The training protocol consisted of a barbell hip thrust exercise . The participants performed 3 sets per 4 repetitions of barbell hip thrust exercise at 85% 1RM . Post activation potentiation using barbell hip thrust exercise was compared with a control condition (a standardised warm-up). The study consisted of three separate sessions. The first session was familiarisation with protocols and equipment. Sessions 2 to 3 were performed in a randomised order. These sessions are separated by a 72-hour washout period. In one of the sessions that was the control session, participants first performed a standardised warm-up and then a 30-metre sprint. In another session, participants first performed a standardised warm-up, then the training protocols, and then, after 4 and 8 minutes, a 30-metre sprint test. Post activation potentiation vs. control reported a meaningful difference for 30-metre sprint time after 4 minutes (p < 0.05) and 8 minutes (p < 0.05). In conclusion, the present outcomes highlight that PAP using a barbell hip thrust bout improves sprint performance in sprinters.